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<DIV class=Section1>
<P class=MsoNormal><FONT face="Times New Roman" size=3><SPAN lang=EN-US
style="FONT-SIZE: 12pt"><IMG id=_x0000_i1025 height=578
src="cid:26B3EAFA72A742BEB046274C37C7170A@PucoMuco"
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<DIV class=Section2>
<P class=MsoNormal><FONT face="Times New Roman" size=6><SPAN lang=EN-US
style="FONT-SIZE: 22pt">Applied Phytoplankton Ecology
Course<o:p></o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT face="Times New Roman" size=3><SPAN lang=EN-US
style="FONT-SIZE: 12pt"><o:p> </o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT face="Times New Roman" size=4><SPAN lang=EN-US
style="FONT-SIZE: 14pt">Marine Science 394 / 394 L (4
credits)</SPAN></FONT><FONT size=6><SPAN lang=EN-US
style="FONT-SIZE: 22pt"><o:p></o:p></SPAN></FONT></P>
<P class=MsoNormal><st1:PlaceType w:st="on"><FONT face="Times New Roman"
size=4><SPAN lang=EN-US
style="FONT-SIZE: 14pt">University</SPAN></FONT></st1:PlaceType><FONT
size=4><SPAN lang=EN-US style="FONT-SIZE: 14pt"> of <st1:PlaceName
w:st="on">Hawaii</st1:PlaceName> at <st1:place w:st="on"><st1:City
w:st="on">Hilo</st1:City></st1:place><o:p></o:p></SPAN></FONT></P>
<P class=MsoNormal><st1:place w:st="on"><st1:City w:st="on"><FONT
face="Times New Roman" size=4><SPAN lang=EN-US
style="FONT-SIZE: 14pt">Hilo</SPAN></FONT></st1:City><FONT size=4><SPAN
lang=EN-US style="FONT-SIZE: 14pt">, <st1:State
w:st="on">Hawaii</st1:State></SPAN></FONT></st1:place><FONT size=4><SPAN
lang=EN-US style="FONT-SIZE: 14pt"><o:p></o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT face="Times New Roman" size=4><SPAN lang=EN-US
style="FONT-SIZE: 14pt">Instructor: Dr. Jason Adolf (<A
href="mailto:jadolf@hawaii.edu">jadolf@hawaii.edu</A>)<o:p></o:p></SPAN></FONT></P>
<P class=MsoNormal><B style="mso-bidi-font-weight: normal"><FONT
face="Times New Roman" size=3><SPAN lang=EN-US
style="FONT-WEIGHT: bold; FONT-SIZE: 12pt; mso-bidi-font-weight: normal">Boat
time includes: New 38’ Force, 18’ o/b Larson, assorted smaller
boats<o:p></o:p></SPAN></FONT></B></P>
<P class=MsoNormal><B style="mso-bidi-font-weight: normal"><FONT
face="Times New Roman" size=3><SPAN lang=EN-US
style="FONT-WEIGHT: bold; FONT-SIZE: 12pt; mso-bidi-font-weight: normal"><o:p> </o:p></SPAN></FONT></B></P>
<P class=MsoNormal><B style="mso-bidi-font-weight: normal"><FONT
face="Times New Roman" size=3><SPAN lang=EN-US
style="FONT-WEIGHT: bold; FONT-SIZE: 12pt; mso-bidi-font-weight: normal">Major
Topics:<o:p></o:p></SPAN></FONT></B></P>
<P class=MsoNormal><FONT face="Times New Roman" size=3><SPAN lang=EN-US
style="FONT-SIZE: 12pt"><o:p> </o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT face="Times New Roman" size=3><SPAN lang=EN-US
style="FONT-SIZE: 12pt">1. <B style="mso-bidi-font-weight: normal"><SPAN
style="FONT-WEIGHT: bold; mso-bidi-font-weight: normal">Phytoplankton culturing
techniques</SPAN></B> are important for establishing and maintaining cultures
that contribute to our understanding of phytoplankton growth and ecology. The
role of microalgae in biotechnology, including <SPAN
class=SpellE>biofuels</SPAN> production, further requires understanding of the
principles of culturing. Students in this class will establish, maintain and
monitor cultures derived from local waters.</SPAN></FONT></P>
<P class=MsoNormal><FONT face="Times New Roman" size=3><SPAN lang=EN-US
style="FONT-SIZE: 12pt"><o:p> </o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT face="Times New Roman" size=3><SPAN lang=EN-US
style="FONT-SIZE: 12pt">2. <B style="mso-bidi-font-weight: normal"><SPAN
style="FONT-WEIGHT: bold; mso-bidi-font-weight: normal">Phytoplankton <SPAN
class=GramE>are</SPAN> used as indicators of water quality</SPAN></B>, but
interpretation requires general knowledge about plankton dynamics, and specific
knowledge of the factors driving local phytoplankton dynamics. Students in this
class will examine phytoplankton dynamics in various coastal Hawaiian settings
and investigate their role as a potential indicator of overall water quality.
Environments include embayments on the leeward and windward sides of <st1:place
w:st="on">Hawaii Island</st1:place> that have very different watershed
characteristics, embayments heavily influenced by submarine groundwater
discharge, and coastal anchialine ponds.</SPAN></FONT></P>
<P class=MsoNormal><FONT face="Times New Roman" size=3><SPAN lang=EN-US
style="FONT-SIZE: 12pt"><o:p> </o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT face="Times New Roman" size=3><SPAN lang=EN-US
style="FONT-SIZE: 12pt">3. <B style="mso-bidi-font-weight: normal"><SPAN
style="FONT-WEIGHT: bold; mso-bidi-font-weight: normal">Flow cytometric analysis
of phytoplankton </SPAN></B>is an important tool for both field and laboratory
work. Students in this class will analyze natural and laboratory samples using
an <SPAN class=SpellE>Accuri</SPAN> C6 analytical flow cytometer and will learn
general principles of flow cytometry as well as specific applications to
investigating phytoplankton dynamics.</SPAN></FONT></P>
<P class=MsoNormal><FONT face="Times New Roman" size=3><SPAN lang=EN-US
style="FONT-SIZE: 12pt"><o:p> </o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT face="Times New Roman" size=3><SPAN lang=EN-US
style="FONT-SIZE: 12pt">4. <B style="mso-bidi-font-weight: normal"><SPAN
style="FONT-WEIGHT: bold; mso-bidi-font-weight: normal">GIS-based mapping of
phytoplankton and water quality</SPAN></B> is important in areas such as coastal
<st1:place w:st="on"><st1:State w:st="on">Hawaii</st1:State></st1:place> where
estuarine gradients affect the distribution of organisms and nutrients in the
near-shore environment. This technique will be applied by students in
<st1:PlaceName w:st="on">Hilo</st1:PlaceName> <st1:PlaceType
w:st="on">Bay</st1:PlaceType> using small boats, and in smaller <st1:place
w:st="on">West Hawaii</st1:place> embayments using kayaks. Data are collected by
‘dataflow’ techniques utilizing YSI V2-4 6600 data <SPAN
class=SpellE>sondes</SPAN>. Students will learn general principles of using
these techniques to study phytoplankton dynamics and water
quality.</SPAN></FONT></P>
<P class=MsoNormal><FONT face="Times New Roman" size=3><SPAN lang=EN-US
style="FONT-SIZE: 12pt"><o:p> </o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT face="Times New Roman" size=3><SPAN lang=EN-US
style="FONT-SIZE: 12pt">5. <B style="mso-bidi-font-weight: normal"><SPAN
style="FONT-WEIGHT: bold; mso-bidi-font-weight: normal">Real-time continuous
monitoring and telemetry from moored platforms</SPAN></B> complements spatial
mapping, providing an important view of temporal changes in conditions. The
<st1:place w:st="on"><st1:PlaceName w:st="on">Hilo</st1:PlaceName>
<st1:PlaceType w:st="on">Bay</st1:PlaceType></st1:place> water quality buoy will
be used to learn about establishment and maintenance of these systems and
analysis of the data provided.</SPAN></FONT></P></DIV></BODY></HTML>